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Proceedings Paper

Combination of SPR and PM-IRRAS for characterization and detection of biosensor arrays
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Paper Abstract

Functionality of biosensor arrays based on SPR imaging is mainly defined by quality of patterned SAM, which is used for sensing of biological substances of interest. The monolayers are commonly not uniform in the micrometer scale. In the current work we describe experimental setups for PM-IRRAS mapping and SPR imaging of self assembly monolayer (SAM) formed by octadecyl phosphonic acid (CH3(CH2)17PO(OH)2) on a patterned Au/Al2O3 surface. By combining highly resolved surface plasmon resonance (SPR) images of the surface with the corresponding PM-IRRAS maps we achieved enhancement of the resolution. In this work we show, that changes of molecular structure in the thin films also induce changes in the SPR signal. Contrary, changes in SPR have their origin in molecular structure within the film.

Paper Details

Date Published: 23 February 2006
PDF: 8 pages
Proc. SPIE 6099, Plasmonics in Biology and Medicine III, 60990I (23 February 2006); doi: 10.1117/12.648360
Show Author Affiliations
Valdas Sablinskas, Vilnius Univ. (Lithuania)
Gerald Steiner, Dresden Univ. of Technology (Germany)
Reiner Salzer, Dresden Univ. of Technology (Germany)

Published in SPIE Proceedings Vol. 6099:
Plasmonics in Biology and Medicine III
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt Gryczynski, Editor(s)

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