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Proceedings Paper

6-DOF control of single living cells by proximal two-beam optical tweezers
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Paper Abstract

We performed a spectroscopy-tomography study of a single living cell to obtain 3-dimensional distribution of proteins in high spatial resolution in real time. In this report, we mention the 6-DOF manipulation of a single living cell to achieve the high spatial resolution 3-dimentional spectrometry. We propose the proximal two- beam optical tweezers as rotational operation. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can operate the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell. And we propose the optical spatial filtering method, paying attention to the diffracted light that is generated from a sample, as translational velocity measurement. This measurement derives the arbitrary component of the spatial frequency from the random refracted index distribution as the periodic light intensity distribution. This periodic light intensity distribution changes in accordance with the translation of an object. Therefore, we can obtain the translational velocity of the non- labeled cell by high-response photodiode.

Paper Details

Date Published: 5 December 2005
PDF: 12 pages
Proc. SPIE 6048, Optomechatronic Actuators and Manipulation, 604804 (5 December 2005); doi: 10.1117/12.648342
Show Author Affiliations
M. Yoshida, Kagawa Univ. (Japan)
I. Ishimaru, Kagawa Univ. (Japan)
K. Ishizaki, Kagawa Univ. (Japan)
Y. Inoue, Kagawa Univ. (Japan)
T. Yasokawa, Kagawa Univ. (Japan)


Published in SPIE Proceedings Vol. 6048:
Optomechatronic Actuators and Manipulation
Kee S. Moon, Editor(s)

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