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Proceedings Paper

Synthesis, metal coordination, and cellular internalization of a siderophore-bearing NIR fluorescent carbocyanine probe
Author(s): Yunpeng Ye; Baogang Xu; Sharon Bloch; Samuel Achilefu
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Paper Abstract

In order to explore novel NIR fluorescent probes for optical imaging in biomedicines, one desferrioxamine (DFO)-bearing NIR fluorescent probe was designed and synthesized based on a dicarboxylic acid-containing carbocyanine (Cypate). Similar to the free DFO, the resulting conjugate Cypate-DFO showed high binding affinity with Fe(III) and Ga(III) as identified by ES-MS. Nevertheless, the iron binding was found to quench its fluorescent emission significantly, suggesting that the siderophore moiety might perturb the spectroscopic properties of the attached carbocyanine fluorophore through metal binding. As observed by fluorescence microscopy, Cypate-DFO showed significant cellular internalization in A549 cells in vitro. Further studies on novel Cypate-DFO derivatives of this type may reveal some exciting properties and biological activities.

Paper Details

Date Published: 14 February 2006
PDF: 5 pages
Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 60970G (14 February 2006); doi: 10.1117/12.648057
Show Author Affiliations
Yunpeng Ye, Washington Univ. School of Medicine, St. Louis (United States)
Baogang Xu, Washington Univ. School of Medicine, St. Louis (United States)
Sharon Bloch, Washington Univ. School of Medicine, St. Louis (United States)
Samuel Achilefu, Washington Univ. School of Medicine, St. Louis (United States)


Published in SPIE Proceedings Vol. 6097:
Optical Molecular Probes for Biomedical Applications
Samuel I. Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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