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Proceedings Paper

Dynamic saturation optical microscopy
Author(s): Jan Sýkora; Thomas Dertinger; Jörg Enderlein
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Paper Abstract

A new concept of fluorescence microscopy is presented allowing the breaking of the diffraction limit of optical microscopy by a factor of ca. five. It relies on measuring the temporal evolution of fluorescence after sudden switch-on of the light excitation. The observed temporal dynamics of the fluorescence signal can be converted into information about the spatial distribution of fluorophores within the exciting laser focus. The proposed scheme is technically simple and versatile, and allows resolution enhancement in all three dimensions.

Paper Details

Date Published: 27 February 2006
PDF: 8 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920E (27 February 2006); doi: 10.1117/12.647987
Show Author Affiliations
Jan Sýkora, Forschungszentrum Jülich (Germany)
Thomas Dertinger, Forschungszentrum Jülich (Germany)
Jörg Enderlein, Forschungszentrum Jülich (Germany)

Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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