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Proceedings Paper

Absorption spectroscopy of individual nano-objects and improved readout of DNA microarrays using photothermal detection
Author(s): Stephane Berciaud; Laurent Cognet; Gerhard A. Blab; David Lasne; José Remacle; Philippe Tamarat; Brahim Lounis
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Paper Abstract

We developed a photothermal method based on scattering around a nano-absorber that allows for the unprecedented detection of individual nano-objects such as gold nanoparticles with diameter down to 1.4 nm as well as CdSe nanocrystals. This method relies on the absorptive properties of the nano-object and does not suffer from the drawbacks of luminescence-based methods. We present here two different applications of this versatile detection method. First, we performed absorption spectroscopy of individual gold nanoparticles as small as 5nm and CdSe nanocrystals in the multiexcitonic regime. Second, we show the applicability of our method for new types of gold nanoparticles based DNA microarrays. In addition to the intrinsic signal stability due to the use of gold labelling, our technique does not require silver staining enhancement and permits to push the signal dynamics of such microarrays from the single nanoparticle detection to almost the full surface coverage.

Paper Details

Date Published: 27 February 2006
PDF: 8 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920A (27 February 2006); doi: 10.1117/12.647808
Show Author Affiliations
Stephane Berciaud, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
Laurent Cognet, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
Gerhard A. Blab, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
David Lasne, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
José Remacle, Eppendorf Array Technologies (Belgium)
Philippe Tamarat, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
Brahim Lounis, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)


Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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