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Proceedings Paper

Single molecule CdSe/ZnS quantum dot and gold nanoparticle detection in live neurons
Author(s): D. Lasne; L. Cognet; S. Berciaud; G. A. Blab; L. Groc; M. Heine; D. Choquet; B. Lounis
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Paper Abstract

We applied single-molecule fluorescence microscopy (using organic dyes or semiconductor quantum dots) to study the lateral diffusion of glutamate receptors (AMPA and NMDA) in live synapses. We directly imaged glutamate receptors movements inside and outside synapses of live cultured hippocampal neurons. We could record exchanges of receptors through lateral diffusion between these different membrane compartments. In addition, our data suggest that this lateral diffusion might be regulated by neuronal activity. To overcome the photobleaching problem inherent to fluorescence techniques we recently developed new optical methods for the detection of individual metallic nanoparticles. We can now detect signatures of diffusing AMPA receptors labeled with 10 nm gold nanoparticles on live neurons.

Paper Details

Date Published: 27 March 2006
PDF: 9 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 609616 (27 March 2006); doi: 10.1117/12.647788
Show Author Affiliations
D. Lasne, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
L. Cognet, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
S. Berciaud, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
G. A. Blab, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)
L. Groc, Lab. de Physiologie Cellulaire de la Synapse, CNRS, Univ. Bordeaux 2 (France)
M. Heine, Lab. de Physiologie Cellulaire de la Synapse, CNRS, Univ. Bordeaux 2 (France)
D. Choquet, Lab. de Physiologie Cellulaire de la Synapse, CNRS, Univ. Bordeaux 2 (France)
B. Lounis, Ctr. de Physique Moléculaire Optique et Hertzienne, CNRS, Univ. Bordeaux 1 (France)


Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin, Editor(s)

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