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Proceedings Paper

Imaging the bone marrow stem cells morphogenesis in PGA scaffold by multiphoton autofluorescence and second harmonic (SHG) imaging
Author(s): Hsuan-Shu Lee; Shu-Wen Teng; Hsiao-Ching Chen; Wen Lo; Yen Sun; Tze-Yu Lin; Ling-Ling Chiou; Ching-Chuan Jiang; Chen-Yuan Dong
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Paper Abstract

The ability to image tissue engineering products without damaging histological procedures is important for the understanding of the dynamics of tissue reorganization and formation. In this work, we test the ability of multiphoton autofluorescence and second harmonic generation microscopy to image engineered tissues following chrondrogenic induction. The system we used is human bone marrow stem cells seeded in the scaffold polyglycolic acid (PGA). Our results show that autofluorescence can be used to image cells while second harmonic generation signal can be used to visualize the synthesis of extracellular matrix. This approach demonstrates the ability of multiphoton imaging in the study of tissue engineering products.

Paper Details

Date Published: 1 March 2006
PDF: 8 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60841C (1 March 2006); doi: 10.1117/12.647607
Show Author Affiliations
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ., College of Medicine (Taiwan)
Shu-Wen Teng, National Taiwan Univ. (Taiwan)
Hsiao-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ., College of Medicine (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Tze-Yu Lin, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ., College of Medicine (Taiwan)
Ching-Chuan Jiang, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ., College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ., College of Medicine (Taiwan)


Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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