Share Email Print
cover

Proceedings Paper

Combined microchannel-type erythrocyte deformability test with optical tweezers
Author(s): Won Gu Lee; Hyunwoo Bang; Junha Park; Seok Chung; Keunchang Cho; Chanil Chung; Dong-Chul Han; Jun Keun Chang
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A combined microchannel-type erythrocyte deformability test with optical tweezers has been developed especially for more sensitive detection of cancerous diseases. To demonstrate the performance and sensitivity of the microchannel-type method, we measured the transit velocity of individual erythrocytes passing through a specific confinement region and calculated the modified elongation index defined by the ratio of the width of the microchannel to the elongated length of the squeezed erythrocytes. To know exactly the effect of optical tweezers on erythrocytes, we investigated several morphologies of optically deformed erythrocytes and measured the shape recovery time of erythrocytes in a static aqueous solution under various powers (~ 24 mW) of 1064-nm laser by a dual-trap optical tweezers. Finally we combined these two methods by considering the key parameters of erythrocyte deformability. The results show that the ambiguity of the overlapped experimental data from microchannel-type erythrocyte deformability test was conspicuously reduced, and that the subtle change (≈ 100-200 ms) in shape recovery time which is one of mechanical properties of erythrocyte membrane surface was remarkably amplified to readily discriminate the difference (≈ 2-3 s) between normal and cancerous blood. This suggests the combined method is more sensitive enough to pinpoint the minor quantitative differences between individual erythrocytes, especially in the field of cancer and cardiovascular diseases.

Paper Details

Date Published: 21 February 2006
PDF: 12 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608813 (21 February 2006); doi: 10.1117/12.647537
Show Author Affiliations
Won Gu Lee, Seoul National Univ. (South Korea)
Hyunwoo Bang, Seoul National Univ. (South Korea)
Junha Park, Seoul National Univ. (South Korea)
Seok Chung, Digital Bio Technology, Inc. (South Korea)
Keunchang Cho, Digital Bio Technology, Inc. (South Korea)
Chanil Chung, Digital Bio Technology, Inc. (South Korea)
Dong-Chul Han, Seoul National Univ. (South Korea)
Jun Keun Chang, Digital Bio Technology, Inc. (South Korea)
Seoul National Univ. (South Korea)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top