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Proceedings Paper

Preliminary micro-Raman images of normal and malignant human skin cells
Author(s): Michael A. Short; Harvey Lui; David I. McLean; Haishan Zeng; Michael X. Chen
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Paper Abstract

Micro-Raman spectroscopy covering a frequency range from 200 to 4000 cm-1 was used to image human skin melanocytes and keratinocytes with a spatial resolution of 0.5 μm. The cells were either cultivated on glass microscope slides or were located within thin sections of skin biopsies mounted on low fluorescence BaF2. A commercially available system was used to obtain the spectra utilizing a x100 long working distance objective with a numerical aperture of 0.8, and a cooled CCD. Both 633 and 515 nm excitations were tried, although the latter proved to be more effcient at producing Raman emission mostly due to the 1/λ4 dependence in light scattering. Fluorescence emission from the cells was surprisingly low. The excitation power at the sample was kept below about 2 mW to avoid damaging the cells; this was the limiting factor on how quickly a Raman image could be obtained. Despite this diffculty we were able to obtain Raman images with rich information about the spectroscopic and structural features within the cytoplasm and cell nuclei. Differences were observed between the Raman images of normal and malignant cells. Spectra from purified DNA, RNA, lipids, proteins and melanin were obtained and these spectra were compared with the skin cell spectra with the aim of understanding how they are distributed over a cell and how the distribution changes between different cells.

Paper Details

Date Published: 27 February 2006
PDF: 6 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930E (27 February 2006); doi: 10.1117/12.647289
Show Author Affiliations
Michael A. Short, Simon Fraser Univ. (Canada)
Harvey Lui, Univ. of British Columbia (Canada)
David I. McLean, Univ. of British Columbia (Canada)
Haishan Zeng, British Columbia Cancer Research Ctr. (Canada)
Michael X. Chen, Simon Fraser Univ. (Canada)


Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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