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Proceedings Paper

The combination of multiphoton and reflected confocal microscopy for cornea imaging
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Paper Abstract

The purpose of this work is to demonstrate the combination of reflective confocal microscopy and multiphoton microscopy and its application in imaging cornea. The difficulty of optically imaging the highly translucent cornea has prevented the development of an effective non-invasive system for the clinical monitoring of the physiological or pathological states of corneas. In this work, we combine reflective confocal microscopy with multiphoton microscopy to demonstrate the potential of our methodology in the minimally invasive imaging of the cornea. The visible reflection signals from cornea can provide structural information of interfaces of different refractive indices while the multiphoton signals generated from the use of near infrared excitation allows deep tissue penetration and reduced photo-damage. In multiphoton imaging, the second harmonic generation (SHG) signal is used to detect collagen in the stroma of the cornea, and the reflective confocal imaging allows detection of the cellular components located in the epithelium. The combination of reflective and multiphoton imaging can be used to reveal complementary structural information of the corneal architecture.. The system is first tested on porcine eye cornea. Assessment of the result on the porcine eye will be used to evaluate the potential of the system as a technique for in vivo clinical applications.

Paper Details

Date Published: 7 March 2006
PDF: 9 pages
Proc. SPIE 6138, Ophthalmic Technologies XVI, 61380M (7 March 2006); doi: 10.1117/12.647225
Show Author Affiliations
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, College of Medicine and College of Engineering (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
Hsin-Yuan Tan, National Taiwan Univ. Hospital (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6138:
Ophthalmic Technologies XVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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