Share Email Print

Proceedings Paper

Effects of low intensity laser irradiation during healing of infected skin lesions in the rat
Author(s): Ethne L. Nussbaum; Lothar Lilge; Tony Mazzulli; Kenneth P.H. Pritzker
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Purpose: To determine the effect of low intensity laser therapy (LILT) on healing of infected skin wounds in the rat. Methods: Wounds on the dorsum of Sprague-Dawley rats (14 per group) were inoculated or sham-inoculated with P. aeruginosa. Wounds were irradiated or sham-irradiated three times weekly from Day 1-19 using 635nm or 808nm diode lasers at radiant exposure of 1 or 20 J/cm2 delivered in continuous wave (CW) or at an intensity modulation frequency of 3800Hz. Wound area and bacterial growth were evaluated three times weekly. Results: CW 808 nm (1 and 20 J/cm2) irradiation generally delayed healing in acute wounds. However, from Day 10 onwards CW 808 nm (1 J/cm2 and 20 J/cm2) and 808 nm 3800 Hz (1 J/cm2) irradiation improved healing in inoculated wounds. Healing in acute wounds improved using 635 nm irradiation at low radiant exposure (1 J/cm2); however, using 635 nm irradiation at high radiant exposure (20 J/cm2) delayed healing. Bacterial balance in wounds was significantly altered using 635 nm (20 J/cm2) and CW 808 nm irradiation (1 and 20 J/cm2). Conclusion: Clearing wounds of normal flora was not associated with improved healing. Proliferation of staphylococcal species in wounds was associated with delayed healing.

Paper Details

Date Published: 28 February 2006
PDF: 7 pages
Proc. SPIE 6140, Mechanisms for Low-Light Therapy, 61400L (28 February 2006); doi: 10.1117/12.647166
Show Author Affiliations
Ethne L. Nussbaum, Mount Sinai Hospital (Canada)
Univ. of Toronto (Canada)
Lothar Lilge, Mount Sinai Hospital (Canada)
Univ. of Toronto (Canada)
Tony Mazzulli, Mount Sinai Hospital (Canada)
Univ. of Toronto (Canada)
Kenneth P.H. Pritzker, Mount Sinai Hospital (Canada)
Univ. of Toronto (Canada)

Published in SPIE Proceedings Vol. 6140:
Mechanisms for Low-Light Therapy
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

© SPIE. Terms of Use
Back to Top