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Proceedings Paper

Application of NIR Raman spectroscopy for detecting and characterizing early dental caries
Author(s): A. C.-T. Ko; L.-P. Choo-Smith; R. Zhu; M. Hewko; C. Dong; B. Cleghorn; M. G. Sowa
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Paper Abstract

Early dental caries detection facilitates implementation of non-surgical methods for arresting caries progression and promoting tooth remineralization. We present a method based on Raman spectroscopy with near-IR laser excitation to provide biochemical contrast for detecting and characterizing incipient carious lesions found in extracted human teeth. Changes in Raman spectra are observed in PO43- vibrations arising from hydroxyapatite of mineralized tooth tissue. Examination of various intensities of the PO43- ν2, ν3, ν4 vibrations showed consistent increased intensities in spectra of carious lesions compared to sound enamel. The spectral changes are attributed to demineralization-induced alterations of enamel crystallite morphology and/or orientation. This hypothesis is supported by reduced Raman polarization anisotropy derived from polarized Raman spectra of carious lesions. Polarized Raman spectral imaging of carious lesions found on whole (i.e. un-sectioned) tooth samples will also be presented.

Paper Details

Date Published: 27 February 2006
PDF: 10 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930L (27 February 2006); doi: 10.1117/12.647159
Show Author Affiliations
A. C.-T. Ko, National Research Council Canada (Canada)
L.-P. Choo-Smith, National Research Council Canada (Canada)
Univ. of Manitoba (Canada)
R. Zhu, National Research Council Canada (Canada)
M. Hewko, National Research Council Canada (Canada)
C. Dong, Univ. of Manitoba (Canada)
B. Cleghorn, Dalhousie Univ. (Canada)
M. G. Sowa, National Research Council Canada (Canada)

Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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