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Proceedings Paper

Glutamate-induced intracellular calcium oscillations in astrocytes with confocal microscopy
Author(s): Yuan Zhang; Wei Zhou; Xiuli Liu; Geng Zhu; Yuxiang Wu; Qingming Luo
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Paper Abstract

Changes in the intracellular Ca2+ concentration ([Ca2+]i) play a crucial role involved in the modulation of signal transduction, development, and plasticity in the CNS. Glial cells can respond to various stimuli with an increase in [Ca2+]i. In this paper, we used confocal microscopy to study calcium transient induced by glutamate in cultured astrocytes. Firstly, 100 μM glutamate induced long-time intracellular calcium oscillations in astrocytes and only a single spike under calcium-free solution. When the concentration of glutamate decreased to 1 μM, only a single spike could be induced. It shows that intracellular calcium oscillations depend on agonist concentration and extracellular Ca2+. Secondly, we investigated amplitude of responses under different stimulation. The amplitude of initial peak induced by 100 μM glutamate decreased in Ca2+-free condition, whereas the duration of kinetics was prolonged. But both the amplitude and area of a single spike induced by 1 μM Glu decreased in Ca2+-free condition. The results show that areaof peak is more accurate than amplitude to display transients of [Ca2+]i. All results above suggest that astrocytes are not passive, they display diverse temporal and spatial increases in [Ca2+]i in response to a variety of stimuli. These [Ca2+]i increases provide a possible means for information coding.

Paper Details

Date Published: 22 February 2006
PDF: 5 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881I (22 February 2006); doi: 10.1117/12.647055
Show Author Affiliations
Yuan Zhang, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)
Xiuli Liu, Huazhong Univ. of Science and Technology (China)
Geng Zhu, Huazhong Univ. of Science and Technology (China)
Yuxiang Wu, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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