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Proceedings Paper

Quasi-confocal fluorescence sectioning with dynamic speckle illumination
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Paper Abstract

We present a new fluorescence microscopy technique that provides depth discrimination in thick tissue. The technique relies on a simple modification to a conventional wide-field microscope, and consists in illuminating the sample with a sequence of speckle patterns and displaying the RMS or the variance of the resultant sequence of fluorescent images. We demonstrate quasi-confocal optical sectioning with an axial resolution of 5 microns FWHM. The lateral resolution is identical to the widefield microscope, namely 0.6 micron FWHM. Images of a mouse brain are compared with standard wide-field images, demonstrating an efficient quasi-sectioning capacity in thick tissue throughout approximatively 100 microns depth. This is achieved because of the high contrast maintained by speckle in a scattering media.

Paper Details

Date Published: 23 February 2006
PDF: 6 pages
Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 60900M (23 February 2006); doi: 10.1117/12.646889
Show Author Affiliations
Cathie Ventalon, Boston Univ. (United States)
Jerome Mertz, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 6090:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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