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Proceedings Paper

Lectin functionalized quantum dots for recognition of mammary tumors
Author(s): Beate S. Santos; Patricia M. A. de Farias; Frederico D. de Menezes; Ricardo de C. Ferreira; Severino A. Júnior; Regina C. B. Q. Figueiredo D.V.M.; Eduardo I. C. Beltrão
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Paper Abstract

In this study we use CdS/Cd(OH)2 quantum dots functionalized with concanavalin-A (Con-A) lectin, specific to glucose/mannose residues, to investigate cell alterations regarding carbohydrate profile in human mammary tissues diagnosed as fibroadenoma (benign tumor). These particles were functionalized with glutaraldehyde and Con-A and incubated with tissue sections of normal and to Fibroadenoma, a benign type of mammary tumor. The tissue sections were deparafinized, hydrated in graded alcohol and treated with a solution of Evans Blue in order to avoid autofluorescence. The fluorescence intensity of QD-Con-A stained tissues showed different patterns, which reflect the carbohydrate expression of glucose/mannose in fibroadenoma when compared to the detection of the normal carbohydrate expression. The pattern of unspecific labeling of the tissues with glutaraldehyde functionalized CdS/Cd(OH)2 quantum dots is compared to the targeting driven by the Con-A lectin. The preliminary findings reported here support the use of CdS/Cd(OH)2 quantum dots as specific probes of cellular alterations and their use in diagnostics.

Paper Details

Date Published: 27 March 2006
PDF: 8 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60961J (27 March 2006); doi: 10.1117/12.646819
Show Author Affiliations
Beate S. Santos, Univ. Federal de Pernambuco (Brazil)
Patricia M. A. de Farias, Univ. Federal de Pernambuco (Brazil)
Frederico D. de Menezes, Univ. Federal de Pernambuco (Brazil)
Ricardo de C. Ferreira, Univ. Federal de Pernambuco (Brazil)
Severino A. Júnior, Univ. Federal de Pernambuco (Brazil)
Regina C. B. Q. Figueiredo D.V.M., Ctr. de Pesquisas Ageu Magalhães (Brazil)
Eduardo I. C. Beltrão, Univ. Federal de Pernambuco (Brazil)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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