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Proceedings Paper

Monitoring activity of living cells marked with colloidal semiconductor quantum dots
Author(s): Patricia M. A. Farias; Beate S. Santos; Frederico D. Menezes; Aluizio G. Brasil, Jr.; Liliane B. Lima; Diego C. N. Silva; Alice A. S. Vieira; Ricardo Ferreira; Adriana Fontes; Diogo Almeida; Luiz C. Barbosa; Carlos L. Cesar
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Paper Abstract

Fluorescent semiconductor nanocrystals in quantum confinement regime (quantum dots) present several well known features which make them very useful tools for biological labeling purposes. Low photo-bleaching rates, high chemical stability, active surface allowing conjugation to living cells, explains the success of this labeling procedure over the commonly used fluorescent dyes. In this paper we report the results obtained with high fluorescent core-shell CdTe-CdS (diameter = 3-7 nm) colloidal nanocrystals synthesized in aqueous medium and conjugated to glucose molecules, incubated with living yeast cells, in order to investigate their glucose up-take activity.

Paper Details

Date Published: 21 February 2006
PDF: 8 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880G (21 February 2006); doi: 10.1117/12.646817
Show Author Affiliations
Patricia M. A. Farias, Univ. Federal de Pernambuco (Brazil)
Beate S. Santos, Univ. Federal de Pernambuco (Brazil)
Frederico D. Menezes, Univ. Federal de Pernambuco (Brazil)
Aluizio G. Brasil, Jr., Univ. Federal de Pernambuco (Brazil)
Liliane B. Lima, Univ. Federal de Pernambuco (Brazil)
Diego C. N. Silva, Univ. Federal de Pernambuco (Brazil)
Alice A. S. Vieira, Univ. Federal de Pernambuco (Brazil)
Ricardo Ferreira, Univ. Federal de Pernambuco (Brazil)
Adriana Fontes, Univ. Estadual de Campinas (Brazil)
Diogo Almeida, Univ. Estadual de Campinas (Brazil)
Luiz C. Barbosa, Univ. Estadual de Campinas (Brazil)
Carlos L. Cesar, Univ. Estadual de Campinas (Brazil)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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