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Proceedings Paper

Determining the gross biochemical composition of cells and tissue with Raman spectrosocpy
Author(s): Judith R. Mourant; Jorge Dominguez; Susan Carpenter; Tamara M. Powers; Anabel Guerra; Kurt W. Short; Nagapratima Kunapareddy; James P. Freyer
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Paper Abstract

The biochemical composition of mammalian cells has been estimated by Raman spectroscopy and the results compared with other biochemical methods. The Raman spectroscopy estimates were performed by fitting measured Raman and infrared spectra of dense cell suspensions to a linear combination of basis components (RNA, DNA, protein, lipid, glycoen). The Raman spectroscopy results are compared to biochemical analyses performed by extraction and quantfication of the biochemical components. Both absolute and relative measurements of biochemical composition are compared. Both the Raman and biochemical results indicate that there are signficant differences in gross biochemical composition dependent on growth stage and tumorigneicity.

Paper Details

Date Published: 27 February 2006
PDF: 9 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930T (27 February 2006); doi: 10.1117/12.646809
Show Author Affiliations
Judith R. Mourant, Los Alamos National Lab. (United States)
Jorge Dominguez, Los Alamos National Lab. (United States)
Susan Carpenter, Los Alamos National Lab. (United States)
Tamara M. Powers, Los Alamos National Lab. (United States)
Anabel Guerra, Los Alamos National Lab. (United States)
Kurt W. Short, Los Alamos National Lab. (United States)
Nagapratima Kunapareddy, Los Alamos National Lab. (United States)
James P. Freyer, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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