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Proceedings Paper

In vivo cell imaging with semiconductor quantum dots and noble metal nanodots
Author(s): Donna J. Arndt-Jovin; M. Arturo Lopez-Quintela; Diane S. Lidke; María J. Rodríguez; Francisco Martinez Santos; Keith A. Lidke; Guy M. Hagen; Thomas M. Jovin
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Paper Abstract

Innovations in fluorescence microscopy of live cells involving new reagents and techniques reveal dynamic processes that were not previously observable and therefore unknown. Water soluble, biofunctionalized semiconductor quantum dots (QDs) provide advantages of much greater photostability compared to conventional fluorescent dyes, and, as a consequence, single QDs can be easily detected. QDs coupled to growth factor ligands behave similarly as the natural ligand and serve as highly fluorescent probes of the erbB family of tyrosine kinase receptors in living cells. Continuous confocal laser scanning microscopy and flow cytometry measurements of QDs combined with visible fluorescent fusions of the receptors have elucidated individual steps in the signaling cascades initiated by these receptors. This report highlights advantages and some disadvantages of QDs, such as size and blinking behavior that complicate some live cell imaging applications. The new class of noble metal nanodots constitute an attractive alternative to QDs in that they are not only highly fluorescent and photostable, but also, much smaller and nontoxic. We present a new synthesis method for the production of Au nanodots. We demonstrate that electrochemical synthesis allows the reproducible control of cluster size. The resulting clusters are more monodisperse than those formed by other methods and are stable over many months. We report their characterization using MALDI-TOF mass spectrometry and UV-VIS spectroscopy.

Paper Details

Date Published: 27 March 2006
PDF: 10 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960P (27 March 2006); doi: 10.1117/12.646794
Show Author Affiliations
Donna J. Arndt-Jovin, Max Planck Institute for Biophysical Chemistry (Germany)
M. Arturo Lopez-Quintela, Univ. of Santiago de Compostela (Spain)
Diane S. Lidke, Max Planck Institute for Biophysical Chemistry (Germany)
Univ. of New Mexico (United States)
María J. Rodríguez, Univ. of Santiago de Compostela (Spain)
Francisco Martinez Santos, Univ. of Santiago de Compostela (Spain)
Keith A. Lidke, Max Planck Institute for Biophysical Chemistry (Germany)
Sandia National Labs. (United States)
Guy M. Hagen, Max Planck Institute for Biophysical Chemistry (Germany)
Thomas M. Jovin, Max Planck Institute for Biophysical Chemistry (Germany)


Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin, Editor(s)

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