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Proceedings Paper

A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy
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Paper Abstract

We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 107 times by a 3-microchannel plate stack. The resulting electron cloud is proximity focused on a cross delay line anode, which allows determining the incident photon position with high accuracy. The imaging and fluorescence lifetime measurement performances of the H33D detector installed on a standard epifluorescence microscope will be presented. We compare them to those of standard single-molecule detectors such as single-photon avalanche photodiode (SPAD) or electron-multiplying camera using model samples (fluorescent beads, quantum dots and live cells). Finally, we discuss the design and applications of future generation of H33D detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

Paper Details

Date Published: 27 February 2006
PDF: 8 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920M (27 February 2006); doi: 10.1117/12.646482
Show Author Affiliations
X. Michalet, Univ. of California/Los Angeles (United States)
O.H.W. Siegmund, Univ. of California/Berkeley (United States)
J. V. Vallerga, Univ. of California/Berkeley (United States)
P. Jelinsky, Univ. of California/Berkeley (United States)
J. E. Millaud, Lawrence Livermore National Lab. (United States)
S. Weiss, Univ. of California/Los Angeles (United States)

Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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