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Proceedings Paper

Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors
Author(s): D. Casanova; D. Giaume; T. Amirtha; T. Gacoin; J.-P. Boilot; A. Alexandrou D.D.S.
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Paper Abstract

We have demonstrated fluorescence resonance energy transfer (FRET) between lanthanide-ion doped oxide nanoparticles acting as donors and organic acceptor molecules (Cy5). Due to the long nanoparticle lifetime and the large Stokes shift between nanoparticle absorption and emission, unambiguous and precise FRET measurements can be performed despite the presence of large free acceptor oncentrations. We determined FRET efficiencies as a function of Cy5 concentration which are in very good agreement with a multiple acceptor-multiple donor calculation.

Paper Details

Date Published: 27 March 2006
PDF: 10 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960J (27 March 2006); doi: 10.1117/12.646479
Show Author Affiliations
D. Casanova, Lab. for Optics and Biosciences, CNRS, INSERM, Ecole Polytechnique (France)
D. Giaume, Lab. of Condensed Matter Physics, CNRS, Ecole Polytechnique (France)
T. Amirtha, Lab. for Optics and Biosciences, CNRS, INSERM, Ecole Polytechnique (France)
T. Gacoin, Lab. of Condensed Matter Physics, CNRS, Ecole Polytechnique (France)
J.-P. Boilot, Lab. of Condensed Matter Physics, CNRS, Ecole Polytechnique (France)
A. Alexandrou D.D.S., Lab. for Optics and Biosciences, CNRS, INSERM, Ecole Polytechnique (France)


Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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