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Proceedings Paper

Imaging of gene expression in vivo with photoacoustic tomography
Author(s): Li Li; Roger J. Zemp; Gina Lungu; George Stoica; Lihong V. Wang
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Paper Abstract

In the post-genomic era, there is an increasing interest in visualizing the expression of functional genes in vivo. With the assistance of the reporter gene technique, various imaging modalities have been adopted for this purpose. In vivo gene expression imaging promises to provide biologists with a powerful tool for deepening our understanding of developmental biology, expanding our knowledge of the genetic basis of disease, and advancing the development of medicine. In this paper, we demonstrate the feasibility of imaging gene expression with photoacoustic imaging, which offers unique absorption contrast with ultrasonic resolution in vivo. We mark tumors in rats with the lacZ reporter gene. The lacZ gene encodes an enzyme β-galactosidase, which yields a dark blue product when acting on a colorimetric assay called X-gal. Photoacoustic tomography at 650nm clearly visualizes the presence of this blue product. The spectroscopic method can also potentially improve specificity. Considering how many staining methods are used in traditional biology, we believe that photoacoustic techniques will revolutionize the field of molecular imaging. The further development of reporter gene systems with high absorbing products in the NIR region is needed.

Paper Details

Date Published: 6 March 2006
PDF: 6 pages
Proc. SPIE 6086, Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 608608 (6 March 2006); doi: 10.1117/12.646322
Show Author Affiliations
Li Li, Texas A&M Univ. (United States)
Roger J. Zemp, Texas A&M Univ. (United States)
Gina Lungu, Texas A&M Univ. (United States)
George Stoica, Texas A&M Univ. (United States)
Lihong V. Wang, Texas A&M Univ. (United States)


Published in SPIE Proceedings Vol. 6086:
Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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