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Proceedings Paper

Rapid Raman spectroscopy of musculoskeletal tissue using a visible laser and an electron-multiplying CCD (EMCCD) detector
Author(s): Kurtulus Golcuk; Gurjit S. Mandair; Andrew F. Callender; William F. Finney; Nadder Sahar; David H. Kohn; Michael D. Morris
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Paper Abstract

Background fluorescence can often complicate the use of Raman microspectroscopy in the study of musculoskeletal tissues. Such fluorescence interferences are undesirable as the Raman spectra of matrix and mineral phases can be used to differentiate between normal and pathological or microdamaged bone. Photobleaching with the excitation laser provides a non-invasive method for reducing background fluorescence, enabling 532 nm Raman hyperspectral imaging of bone tissue. The signal acquisition time for a 400 point Raman line image is reduced to 1-4 seconds using electronmultiplying CCD (EMCCD) detector, enabling acquisition of Raman images in less than 10 minutes. Rapid photobleaching depends upon multiple scattering effects in the tissue specimen and is applicable to some, but not all experimental situations.

Paper Details

Date Published: 27 February 2006
PDF: 7 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 609314 (27 February 2006); doi: 10.1117/12.646209
Show Author Affiliations
Kurtulus Golcuk, Univ. of Michigan (United States)
Gurjit S. Mandair, Univ. of Michigan (United States)
Andrew F. Callender, Univ. of Michigan (United States)
William F. Finney, Univ. of Michigan (United States)
Nadder Sahar, Univ. of Michigan (United States)
David H. Kohn, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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