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Proceedings Paper

SPR dispersion compensation for a gold-coated corrugated surface
Author(s): Michael T. Reilly Sr.; Marsela Jorgolli; Peter A. Nessing; Ernest F. Guignon; Salvador M. Fernandez
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Paper Abstract

The objective of this effort is to improve upon a microarray-based system for label-free, high-throughput proteomic analysis. The system operation is based on a novel grating-coupled surface plasmon resonance imaging (GCSPRI) technology that enables measurement of hundreds to possibly thousands of binding events simultaneously without the limitations of reporter molecules. We expect that this technology will provide a powerful new tool for highly multiplexed analysis of an organism's global protein profile, reflecting metabolic and physiologic activity in relation to time, development and interaction with the environment. It will be applicable to the detection of a broad range of metabolic products, signaling molecules, hormones, enzymes, receptors and other proteins. We present improvements by way of SPR-dispersion compensation with a properly oriented transmission grating placed in the excitation path. This illumination technique is broad-band which reduces the effects of thin-film interference, permitting a 20-fold decrease in sample volume as well as providing more interrogation light.

Paper Details

Date Published: 23 February 2006
PDF: 11 pages
Proc. SPIE 6099, Plasmonics in Biology and Medicine III, 60990H (23 February 2006); doi: 10.1117/12.646189
Show Author Affiliations
Michael T. Reilly Sr., Ciencia, Inc. (United States)
Marsela Jorgolli, Ciencia, Inc. (United States)
Peter A. Nessing, Ciencia, Inc. (United States)
Ernest F. Guignon, Ciencia, Inc. (United States)
Salvador M. Fernandez, Ciencia, Inc. (United States)

Published in SPIE Proceedings Vol. 6099:
Plasmonics in Biology and Medicine III
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt Gryczynski, Editor(s)

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