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Proceedings Paper

Dynamics of cells function on laser cell-chip system
Author(s): Toshihiro Kushibiki; Tomoko Sano; Katsunori Ishii; Sachiko Yoshihashi-Suzuki; Kunio Awazu
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Paper Abstract

A new type of cell-cultivation system based on laser processing has been developed for the on-chip cultivation of living cells. We introduce a "laser cell-chip", on which migration of cells, such as stem cells, tumor cells or immunocompetent cells, can be observed. A sheet prepared from epoxy resin was processed by KrF excimer laser (248 nm, 1.6 J/cm2) for preparation of microgrooved surfaces with various groove width, spacing, and depth. A laser cell-chip can make kinetic studies of cell migration depending on the concentration gradient of a chemoattractant. In this study, megakaryocytes were used for the migration on a groove of laser cell-chip by the concentration gradient of the stromal cell derived factor 1 (SDF-1/CXCL12). SDF-1/CXCL12 plays an important and unique role in the regulation of stem/progenitor cell trafficking. A megakaryocyte was migrated on a groove of laser cell-chip depending on the optical concentration gradient of SDF-1/CXCL12. Since SDF-1/CXCL12-induced migration of mature megakaryocyte was known to increase the platelet production in the bone marrow extravascular space, the diagnosis of cell migration on laser cell-chip could provide a new strategy to potentially reconstitute hematopoiesis and avoid life-threatening hemorrhage after myelosuppression or bone marrow failure.

Paper Details

Date Published: 25 February 2006
PDF: 6 pages
Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 60800B (25 February 2006); doi: 10.1117/12.646188
Show Author Affiliations
Toshihiro Kushibiki, Osaka Univ. (Japan)
Tomoko Sano, Osaka Univ. (Japan)
Katsunori Ishii, Osaka Univ. (Japan)
Sachiko Yoshihashi-Suzuki, Osaka Univ. (Japan)
Kunio Awazu, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 6080:
Advanced Biomedical and Clinical Diagnostic Systems IV
Gerald E. Cohn; Warren S. Grundfest M.D.; David A. Benaron M.D.; Tuan Vo-Dinh, Editor(s)

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