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Proceedings Paper

Optical trapping investigation on the effects of salicylate on electromechanical properties of plasma membranes
Author(s): Linda Lee; Feng Qian; William E. Brownell; Bahman Anvari
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Paper Abstract

The ability of cellular membranes to generate electrically-induced mechanical force (EMF) has been demonstrated in many cell types, including cochlear outer hair cells, axons, and some cultured mammalian cells. Models of membrane based EMF generation are based on an interaction between the transmembrane electric field and membrane surface charge. We use a technique that combines optical trapping with voltage clamping to investigate the effects of an electrically charged amphipathic agent on EMF by membrane tethers. Our preliminary results indicate that salicylate, a negatively charged amphipathic agent, which is also known to cause reversible hearing loss and reduce outer hair cell electromotility, reduces EMF in membrane tethers. These measurements provide a basis to better understand the role of membrane charge properties in EMF generation.

Paper Details

Date Published: 21 February 2006
PDF: 6 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881A (21 February 2006); doi: 10.1117/12.646130
Show Author Affiliations
Linda Lee, Baylor College of Medicine (United States)
Feng Qian, Rice Univ. (United States)
William E. Brownell, Baylor College of Medicine (United States)
Bahman Anvari, Rice Univ. (United States)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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