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Proceedings Paper

Three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope
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Paper Abstract

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

Paper Details

Date Published: 23 February 2006
PDF: 8 pages
Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 60900A (23 February 2006); doi: 10.1117/12.645962
Show Author Affiliations
G. Aoki, Univ. of Tsukuba (Japan)
M. Itoh, Univ. of Tsukuba (Japan)
Y. Yasuno, Univ. of Tsukuba (Japan)
T. Yatagai, Univ. of Tsukuba (Japan)


Published in SPIE Proceedings Vol. 6090:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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