Share Email Print
cover

Proceedings Paper

Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo
Author(s): Takashi Sakurai; Seiji Yamamoto; Atsuo Miyakawa; Yoshihiko Wakazono; Takato O. Yoshida; Eiji Kohno; Remi Tsuchiya-Susuki; Yong Wang; Hideo Hirukawa; Susumu Terakawa
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

To study cellular morphology and functions in vivo in realtime, we developed a fiber-coupled confocal microscope (FCM), and observed fluorescently-labeled cells inside the body of anesthetized rat. We developed an imaging fiber bundle (IFB), which consisted of an objective lens and a multi-fiber assembly (unit fiber: NA > 0.4, 3 micron in diameter). By combining the IFB with a real-time confocal scanner, we detected intracellular signals of the molecular messenger, and the death signals in the form of fluorescence changes even from cells located deep (> 2 mm) inside the solid organs. The FCM we developed is very promising for detailed studies in both the cell-based researches and clinical researches.

Paper Details

Date Published: 16 February 2006
PDF: 6 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608803 (16 February 2006); doi: 10.1117/12.645939
Show Author Affiliations
Takashi Sakurai, Hamamatsu Univ. School of Medicine (Japan)
Seiji Yamamoto, Hamamatsu Univ. School of Medicine (Japan)
Atsuo Miyakawa, Hamamatsu Univ. School of Medicine (Japan)
Yoshihiko Wakazono, Hamamatsu Univ. School of Medicine (Japan)
Takato O. Yoshida, Hamamatsu Univ. School of Medicine (Japan)
Eiji Kohno, Hamamatsu Univ. School of Medicine (Japan)
Remi Tsuchiya-Susuki, Hamamatsu Univ. School of Medicine (Japan)
Yong Wang, Hamamatsu Univ. School of Medicine (Japan)
Hideo Hirukawa, Yokogawa Electric Co. (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top