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Proceedings Paper

Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage
Author(s): Miya Ishihara; Masato Sato; Nagatoshi Kaneshiro; Genya Mitani; Shunichi Sato; Masayuki Ishihara; Joji Mochida; Makoto Kikuchi
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Paper Abstract

We demonstrated the capability of a photoacoustic method for viscoelastic measurement. The measurement method has already proved to be useful for evaluation of regenerative medicine of articular cartilage. However, characterization of the extracellular matrix as well as determination of the viscoelastic property should be carried out for evaluation of regenerative medicine because the extracellular matrix plays an important role. We therefore developed a method for characterization of the extracellular matrix that can be performed simultaneously with the photoacoustic measurement. Since collagen molecules, which are the major contents of the cartilage extracellular matrix, are well known as endogenous fluorescent molecules, it is possible that fluorescence measurement will enable characterization of the extracellular matrix. Third harmonic Q-switched Nd:YAG laser pulses were used as an excitation light source. The time-resolved fluorescence spectroscopy was obtained by using a photonic multi-channel analyzer. Tissue-engineered cartilages cultured under different conditions for various periods were used as samples. Different culture conditions resulted in different extracellular matrix formations. There were significant differences in the measured fluorescent parameters among the culture conditions of cartilage because chondrocytes produce a specific extracellular matrix depending on its culture condition. The specific extracellular matrix contained a specific type of collagen such as collagen type I or type II, which each have specific fluorescent features. Thus, the fluorescent parameters enabled characterization of synthesis of cartilage-associated extracellular matrix. Therefore, the combination of fluorescence and photoacoustic measurement is expected to become a useful evaluation method in regenerative medicine.

Paper Details

Date Published: 1 March 2006
PDF: 4 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840V (1 March 2006); doi: 10.1117/12.645888
Show Author Affiliations
Miya Ishihara, National Defense Medical College (Japan)
Masato Sato, Tokai Univ. School of Medicine (Japan)
Nagatoshi Kaneshiro, Tokai Univ. School of Medicine (Japan)
Genya Mitani, Tokai Univ. School of Medicine (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Masayuki Ishihara, National Defense Medical College Research Institute (Japan)
Joji Mochida, Tokai Univ. School of Medicine (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)


Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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