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Proceedings Paper

In vivo analysis of tissue by Raman microprobe: examination of human skin lesions and esophagus Barrett’s mucosa on an animal model
Author(s): Ali Tfayli; Olivier Piot; Sylvie Derancourt; Guillaume Cadiot; Marie Danielle Diebold; Philippe Bernard; Michel Manfait
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Paper Abstract

In the last few years, Raman spectroscopy has been increasingly used for the characterization of normal and pathological tissues. A new Raman system, constituted of optic fibers bundle coupled to an axial Raman spectrometer (Horiba Jobin Yvon SAS), was developed for in vivo investigations. Here, we present in vivo analysis on two tissues: human skin and esophagus mucosa on a rat model. The skin is a directly accessible organ, representing a high diversity of lesions and cancers. Including malignant melanoma, basal cell carcinoma and the squamous cell carcinoma, skin cancer is the cancer with the highest incidence worldwide. Several Raman investigations were performed to discriminate and classify different types of skin lesions, on thin sections of biopsies. Here, we try to characterize in vivo the different types of skin cancers in order to be able to detect them in their early stages of development and to define precisely the exeresis limits. Barrett's mucosa was also studied by in vivo examination of rat's esophagus. Barrett's mucosa, induced by gastro-esophageal reflux, is a pretumoral state that has to be carefully monitored due to its high risk of evolution in adenocarcinoma. A better knowledge of the histological transformation of esophagus epithelium in a Barrett's type will lead to a more efficient detection of the pathology for its early diagnosis. To study these changes, an animal model (rats developing Barrett's mucosa after duodenum - esophagus anastomosis) was used. Potential of vibrational spectroscopy for Barrett's mucosa identification is assessed on this model.

Paper Details

Date Published: 27 February 2006
PDF: 10 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 609312 (27 February 2006); doi: 10.1117/12.645874
Show Author Affiliations
Ali Tfayli, Unite MeDIAN, CNRS (France)
Olivier Piot, Unite MeDIAN, CNRS (France)
Sylvie Derancourt, CHU Robert Debre (France)
Guillaume Cadiot, CHU Robert Debre (France)
Marie Danielle Diebold, CHU Robert Debre (France)
Philippe Bernard, CHU Robert Debre (France)
Michel Manfait, Unite MeDIAN, CNRS (France)

Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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