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Proceedings Paper

New fluorescent chemosensors for magnesium ions in living cells
Author(s): L. Prodi; L. S. Dolci; M. Montalti; N. Zaccheroni; G. Farruggia; G. Andreani; E. Carpenè; S. Iotti; P. B. Savage; V. Trapani; P. Sale; F. I. Wolf
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Paper Abstract

Although magnesium ions play a key role in many fundamental biological processes, information about its intracellular regulation is still scarce, due to the lack of appropriate detection methods. Here, we report the spectroscopic characterization of two diaza-18-crown-6 hydroxyquinoline derivatives (DCHQ) and we propose their application for the determination of total Mg2+ concentration and in confocal imaging as effective Mg2+ indicators. DCHQ derivatives 1 and 2 bind Mg2+ with much higher affinity than other available probes (Kd = 44 and 73 mM, respectively) with a concomitant strong fluorescence increase. On the other hand, the fluorescence intensity is not significantly affected by other divalent cations, most importantly Ca2+, or by pH changes within the physiological range. Evidence is provided on the use of fluorometric data to derive totalcellular Mg2+content, which is in agreement with atomic absorption data. Furthermore, we show that DCHQ compounds can be effectively employed to map intracellular ion distribution and movements in live cells by confocal microscopy. These findings suggest that DCHQ derivatives may serve as new probes for the study of Mg2+ regulation, allowing sensitive and straightforward detection of both static and dynamic signals.

Paper Details

Date Published: 14 February 2006
PDF: 7 pages
Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 60970C (14 February 2006); doi: 10.1117/12.645783
Show Author Affiliations
L. Prodi, Univ. degli Studi di Bologna (Italy)
L. S. Dolci, Univ. degli Studi di Bologna (Italy)
M. Montalti, Univ. degli Studi di Bologna (Italy)
N. Zaccheroni, Univ. degli Studi di Bologna (Italy)
G. Farruggia, Univ. degli Studi di Bologna (Italy)
G. Andreani, Univ. degli Studi di Bologna (Italy)
E. Carpenè, Univ. degli Studi di Bologna (Italy)
S. Iotti, Univ. degli Studi di Bologna (Italy)
P. B. Savage, Brigham Young Univ. (United States)
V. Trapani, Univ. Cattolica del Sacro Cuore (Italy)
P. Sale, Univ. Cattolica del Sacro Cuore (Italy)
F. I. Wolf, Univ. Cattolica del Sacro Cuore (Italy)


Published in SPIE Proceedings Vol. 6097:
Optical Molecular Probes for Biomedical Applications
Samuel I. Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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