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Proceedings Paper

Frame self-division applied in analysis of intrinsic signal optical imaging data
Author(s): Weihua Luo; Pengcheng Li; Li Zhang; Xiaohua Lv; Shaoqun Zeng; Qingming Luo
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Paper Abstract

In intrinsic signal optical imaging data, the response signal related cortical activity is very small compared to the background signal intensity and accompanied with unwanted noise from several different sources. Although there are some sophisticated data analysis techniques developed to achieve this critical demand, experience proved that no method perfect enough to be optimal for all experimental situations. Here we introduced a simple and practicable pre-processing method to attenuate the global noise source, such as the fluctuation of the illuminating light, changes of the animal's blood pressure and vasomotor oscillations. Before image processing to characterize the spatiotemporal response from cortical activity, each frame of image data was divided by the average or median of the current frame data. We applied the method to both simulated data and experimental optical imaging data to evaluate the validity and limitation.

Paper Details

Date Published: 3 March 2006
PDF: 8 pages
Proc. SPIE 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III, 60850E (3 March 2006); doi: 10.1117/12.645570
Show Author Affiliations
Weihua Luo, Huazhong Univ. of Science and Technology (China)
Pengcheng Li, Huazhong Univ. of Science and Technology (China)
Li Zhang, Huazhong Univ. of Science and Technology (China)
Xiaohua Lv, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6085:
Complex Dynamics and Fluctuations in Biomedical Photonics III
Valery V. Tuchin, Editor(s)

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