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Proceedings Paper

Live cell imaging of the endocytosis and the intracellular trafficking of multifunctional lipid nanoparticles
Author(s): Tieqiao Zhang; S. Narasimhan Danthi; Jianwu Xie; Dehong Hu; Peter Lu; King Li
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Paper Abstract

Artificial lipid nanoparticles have drawn great attention due to their potential in medicine. Linked with targeting ligands, they can be used as probes and/or gene delivery vectors for specific types of target cells. Therefore, they are very promising agents in early detection, diagnosis and treatment of cancers and other genetic diseases. However, there are several barriers blocking the applications. Controlling the cellular uptake of the lipid nanoparticles is an important technical challenge to overcome. Understanding the mechanism of the endocytosis and the following intracellular trafficking is very important for improving the design and therefore the efficiency as a drug delivery system. By using fluorescence microscopy methods, we studied the endocytosis of lipid nanoparticles by live M21 cells. The movements of the nanoparticles inside the cell were quantitatively characterized and classified based on the diffusion behavior. The trajectories of nanoparticles movement over the cell membrane revealed hop-diffusion behavior prior to the endocytosis. Fast movement in large steps is observed in intracellular trafficking and is attributed to active movement along microtubule. These observations help to understand the mechanism of the endocytosis and the pathway of the particles in cells.

Paper Details

Date Published: 28 February 2006
PDF: 4 pages
Proc. SPIE 6095, Nanobiophotonics and Biomedical Applications III, 60950D (28 February 2006); doi: 10.1117/12.645398
Show Author Affiliations
Tieqiao Zhang, National Institutes of Health (United States)
S. Narasimhan Danthi, National Institutes of Health (United States)
Jianwu Xie, National Institutes of Health (United States)
Dehong Hu, Pacific Northwest National Lab. (United States)
Peter Lu, Pacific Northwest National Lab. (United States)
King Li, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 6095:
Nanobiophotonics and Biomedical Applications III
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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