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Proceedings Paper

Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging
Author(s): Jamshid Temirov; Andrew Bradbury; James Werner
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Paper Abstract

In this work we report Single Molecule (SM) microscopy studies of binding events between individual surface immobilized antibodies and fluorescent antigens using Total Internal Reflection (TIR) microscopy. Specific binding events of single biotin-conjugated 1quantum dots to anti-biotin antibodies, immobilized on an amine-coated cover glass using a heterobifunctional photo-reactive cross-linker were observed. The methodology of calculating the binding affinity (dissociation constant) from the time series of 2-D images is described.

Paper Details

Date Published: 27 February 2006
PDF: 10 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920O (27 February 2006); doi: 10.1117/12.645334
Show Author Affiliations
Jamshid Temirov, Los Alamos National Lab. (United States)
Andrew Bradbury, Los Alamos National Lab. (United States)
James Werner, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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