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Proceedings Paper

Chitosan adhesive for laser tissue repair
Author(s): A. Lauto; M. Stoodley; A. Avolio; L. J. R. Foster
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Paper Abstract

Background. Laser tissue repair usually relies on haemoderivate solders, based on serum albumin. These solders have intrinsic limitations that impair their widespread use, such as limited repair strength, high solubility, brittleness and viral transmission. Furthermore, the solder activation temperature (65-70 °C) can induce significant damage to tissue. In this study, a new laser-activated biomaterial for tissue repair was developed and tested in vitro and in vivo to overcome some of the shortcomings of traditional solders. Materials and Methods. Flexible and insoluble strips of chitosan adhesive (surface area ~34 mm2, thickness ~20 μm) were developed and bonded on sheep intestine with a laser fluence and irradiance of 52 ± 2 J/cm2 and ~15 W/cm2 respectively. The temperature between tissue and adhesive was measured using small thermocouples. The strength of repaired tissue was tested by a calibrated tensiometer. The adhesive was also bonded in vivo to the sciatic nerve of rats to assess the thermal damage induced by the laser (fluence = 65 ± 11 J/cm2, irradiance = 15 W/cm2) four days post-operatively. Results. Chitosan adhesives successfully repaired intestine tissue, achieving a repair strength of 0.50 ± 0.15 N (shear stress = 14.7 ± 4.7 KPa, n=30) at a temperature of 60-65 °C. The laser caused demyelination of axons at the operated site; nevertheless, the myelinated axons retained their normal morphology proximally and distally.

Paper Details

Date Published: 22 February 2006
PDF: 13 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60780X (22 February 2006); doi: 10.1117/12.645155
Show Author Affiliations
A. Lauto, Univ. of New South Wales (Australia)
The Univ. of Western Australia (Australia)
M. Stoodley, Univ. of New South Wales (Australia)
A. Avolio, Univ. of New South Wales (Australia)
L. J. R. Foster, Univ. of New South Wales (Australia)


Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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