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Proceedings Paper

In vivo molecular and morphological imaging by real time confocal mini-microscopy
Author(s): Martin Goetz; Sebastian Gregor; Christian Fottner; Jose Garcia-Lazaro; Esther Schirrmacher; Oliver Kempski; Peter Bartenstein; Mathias Weber; Stefan Biesterfeld; Peter R. Galle; Markus F. Neurath; Ralf Kiesslich
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Paper Abstract

We evaluated a newly developed miniaturized confocal laser microscopy probe for real-time in vivo molecular and morphological imaging of normal, inflammatory, and malignant tissue in rodents. In the rigid mini-microscopy probe (diameter 7 mm), a single line laser delivers an excitation wavelength of 488 nm. Optical slice thickness is 7 μm, lateral resolution 0.7 μm. The range of the z-axis is 0 - 250 μm below the tissue surface. Organ systems were examined in vivo in rodent models of human diseases. FITC-labeled Lycopersion esculentum lectin was injected or selected cell populations stained for molecular targeting. Morphological imaging was performed using fluorescein sodium, FITC-labeled dextran, and/or acriflavine hydrochloride. Cellular and subcellular details could be readily visualised in vivo at high resolution. Tissue characteristics of different organs were rendered at real time. Selective blood cell staining allowed observation of blood flow and cell migration. Inflammatory diseases such as hepatitis were diagnosed, and tumors were characterized under microscopic control in vivo. Confocal mini-microscopy allows real time in vivo molecular and morphological histologic imaging at high resolution of normal and diseased tissue. Since confocal microscopy is applicable to humans, this technology will have a high impact on different faculties in medicine.

Paper Details

Date Published: 23 February 2006
PDF: 8 pages
Proc. SPIE 6082, Endoscopic Microscopy, 60820G (23 February 2006); doi: 10.1117/12.645078
Show Author Affiliations
Martin Goetz, Univ. of Mainz (Germany)
Sebastian Gregor, Univ. of Mainz (Germany)
Christian Fottner, Univ. of Mainz (Germany)
Jose Garcia-Lazaro, Univ. of Mainz (Germany)
Esther Schirrmacher, Univ. of Mainz (Germany)
Oliver Kempski, Univ. of Mainz (Germany)
Peter Bartenstein, Univ. of Mainz (Germany)
Mathias Weber, Univ. of Mainz (Germany)
Stefan Biesterfeld, Univ. of Mainz (Germany)
Peter R. Galle, Univ. of Mainz (Germany)
Markus F. Neurath, Univ. of Mainz (Germany)
Ralf Kiesslich, Univ. of Mainz (Germany)

Published in SPIE Proceedings Vol. 6082:
Endoscopic Microscopy
Guillermo J. Tearney M.D.; Thomas D. Wang, Editor(s)

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