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Proceedings Paper

Real-time imaging of tissue microstructures using intrinsic optical signatures
Author(s): Bevin Lin; Chad A. Lieber; Jason T. Fitzgerald; Andromachi P. Michalopoulou; Rajesh N. Raman; Christopher D. Pivetti; Christoph Troppmann; Dennis L. Matthews; Stavros G. Demos
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Paper Abstract

We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

Paper Details

Date Published: 22 February 2006
PDF: 6 pages
Proc. SPIE 6091, Optical Biopsy VI, 60910G (22 February 2006); doi: 10.1117/12.644954
Show Author Affiliations
Bevin Lin, Univ. of California Davis (United States)
Chad A. Lieber, Univ. of California Davis (United States)
Jason T. Fitzgerald, Univ. of California/Davis (United States)
Andromachi P. Michalopoulou, Univ. of California/Davis (United States)
Rajesh N. Raman, Univ. of California Davis (United States)
Christopher D. Pivetti, Univ. of California Davis Medical Ctr. (United States)
Christoph Troppmann, Univ. of California Davis Medical Ctr. (United States)
Dennis L. Matthews, Univ. of California Davis Ctr. for Biophotonics (United States)
Lawrence Livermore National Lab. (United States)
Stavros G. Demos, Univ. of California Davis Medical Ctr. (United States)
Lawrence Livermore National Lab. (United States)


Published in SPIE Proceedings Vol. 6091:
Optical Biopsy VI
Robert R. Alfano; Alvin Katz, Editor(s)

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