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Proceedings Paper

Characteristics of a dual-detector charge coupled device (CCD) based optical fluorescence imaging system
Author(s): Jason Y. Chen; Yuhua Li; Hong Liu
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Paper Abstract

A unique dual-detector charge coupled device (CCD) system has been developed and it has been previously shown to possess a high temporal resolution and the capability to perform ratio imaging on gel phantoms. We used this CCD system to analyze differences in fluorescence intensity ratios at different levels of pH. We found that the ratio of emissions at 532 nm and 635 nm was ~3.8 in gels of pH 6.1, corresponding to cancerous tissue, and ~3.5 in gels of pH 7.2, corresponding to normal tissue. In addition, the accuracy of these intensity results was verified by calculating noise and dark current. We found that dark current intensity never exceeded 1 arbitrary unit, which is a negligible percentage of the intensity levels of the gel phantoms. Our system is very capable of accurately performing certain biomedical experiments, such as cancer detection.

Paper Details

Date Published: 3 March 2006
PDF: 6 pages
Proc. SPIE 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III, 60850M (3 March 2006); doi: 10.1117/12.644810
Show Author Affiliations
Jason Y. Chen, Univ. of Oklahoma (United States)
Yuhua Li, Univ. of Oklahoma (United States)
Hong Liu, Univ. of Oklahoma (United States)

Published in SPIE Proceedings Vol. 6085:
Complex Dynamics and Fluctuations in Biomedical Photonics III
Valery V. Tuchin, Editor(s)

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