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Proceedings Paper

Fully integrated multiplexed lab-on-a-card assay for enteric pathogens
Author(s): B. H. Weigl; J. Gerdes; P. Tarr; P. Yager; L. Dillman; R. Peck; S. Ramachandran; M. Lemba; M. Kokoris; M. Nabavi; F. Battrell; D. Hoekstra; E. J. Klein; D. M. Denno
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Paper Abstract

Under this NIH-funded project, we are developing a lab-on-a-card platform to identify enteric bacterial pathogens in patients presenting with acute diarrhea, with special reference to infections that might be encountered in developing countries. Component functions that are integrated on this platform include on-chip immunocapture of live or whole pathogens, multiplexed nucleic acid amplification and on-chip detection, sample processing to support direct use of clinical specimens, and dry reagent storage and handling. All microfluidic functions are contained on the lab card. This new diagnostic test will be able to rapidly identify and differentiate Shigella dysenteriae serotype 1, Shigella toxin-producing Escherichia coli, E. coli 0157, Campylobacter jejuni, and Salmonella and Shigella species. This presentation will report on progress to date on sample and bacteria processing methodologies, identification and validation of capture antibodies and strategy for organism immunocapture, identification and validation of specific polymerase chain reaction (PCR) primer sequences for over 200 clinical isolates of enteric pathogens, and implementation of on-chip nucleic acid extraction for a subset of those pathogens.

Paper Details

Date Published: 23 January 2006
PDF: 11 pages
Proc. SPIE 6112, Microfluidics, BioMEMS, and Medical Microsystems IV, 611202 (23 January 2006); doi: 10.1117/12.644714
Show Author Affiliations
B. H. Weigl, Program for Appropriate Technology in Health (United States)
J. Gerdes, Micronics, Inc. (United States)
P. Tarr, Washington Univ. (United States)
P. Yager, Univ. of Washington (United States)
L. Dillman, Program for Appropriate Technology in Health (United States)
R. Peck, Program for Appropriate Technology in Health (United States)
S. Ramachandran, Univ. of Washington (United States)
M. Lemba, Univ. of Washington (United States)
M. Kokoris, Micronics, Inc. (United States)
M. Nabavi, Micronics, Inc. (United States)
F. Battrell, Micronics, Inc. (United States)
D. Hoekstra, Micronics, Inc. (United States)
E. J. Klein, Univ. of Washington (United States)
D. M. Denno, Univ. of Washington (United States)


Published in SPIE Proceedings Vol. 6112:
Microfluidics, BioMEMS, and Medical Microsystems IV
Ian Papautsky; Wanjun Wang, Editor(s)

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