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Proceedings Paper

Light-scattering spectroscopic optical coherence tomography for differentiating cells in 3D cell culture
Author(s): Chenyang Xu; P. Scott Carney; Wei Tan; Stephen A. Boppart
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Paper Abstract

Combining light scatting spectroscopy (LSS) and spectroscopic optical coherence tomography (LS-SOCT) can provide a wealth of information. A theoretical model for analyzing the scattering in SOCT is proposed based on plane wave decomposition. Based on the model, we discuss the possibility of matching the physical characteristics of scatterers with observed spectroscopic signals. Many complicating factors are considered including the effects of scatterer size, incident light polarization, interference between the fields scattered from closely adjacent scatterers, and the numerical aperture of the OCT system. We found the modulation of the spectrum of the incident light by scattering of a plane wave from a single sphere is a good indicator of particle size and composition. We demonstrate that measuring wavelength-dependent scattering in SOCT can be used for particle sizing and contrast enhancement by differentiating cells in 3D cell culture.

Paper Details

Date Published: 20 February 2006
PDF: 10 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608804 (20 February 2006); doi: 10.1117/12.644415
Show Author Affiliations
Chenyang Xu, Univ. of Illinois at Urbana-Champaign (United States)
P. Scott Carney, Univ. of Illinois at Urbana-Champaign (United States)
Wei Tan, Univ. of Illinois at Urbana-Champaign (United States)
Stephen A. Boppart, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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