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Proceedings Paper

Raman spectroscopic biochemical mapping of tissues
Author(s): Nicholas Stone; Maria Consuelo Hart Prieto; Catherine Ann Kendall; Geeta Shetty; Hugh Barr
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Paper Abstract

Advances in technologies have brought us closer to routine spectroscopic diagnosis of early malignant disease. However, there is still a poor understanding of the carcinogenesis process. For example it is not known whether many cancers follow a logical sequence from dysplasia, to carcinoma in situ, to invasion. Biochemical tissue changes, triggered by genetic mutations, precede morphological and structural changes. These can be probed using Raman or FTIR microspectroscopy and the spectra analysed for biochemical constituents. Local microscopic distribution of various constituents can then be visualised. Raman mapping has been performed on a number of tissues including oesophagus, breast, bladder and prostate. The biochemical constituents have been calculated at each point using basis spectra and least squares analysis. The residual of the least squares fit indicates any unfit spectral components. The biochemical distribution will be compared with the defined histopathological boundaries. The distribution of nucleic acids, glycogen, actin, collagen I, III, IV, lipids and others appear to follow expected patterns.

Paper Details

Date Published: 27 February 2006
PDF: 9 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930U (27 February 2006); doi: 10.1117/12.644178
Show Author Affiliations
Nicholas Stone, Gloucestershire Royal Hospital (United Kingdom)
Maria Consuelo Hart Prieto, Gloucestershire Royal Hospital (United Kingdom)
Catherine Ann Kendall, Gloucestershire Royal Hospital (United Kingdom)
Geeta Shetty, Gloucestershire Royal Hospital (United Kingdom)
Hugh Barr, Gloucestershire Royal Hospital (United Kingdom)


Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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