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Proceedings Paper

Antioxidant assay using genetically engineered bioluminescent Escherichia coli
Author(s): Amelita Bartolome; Bernadette Macalino; Ian Lemuel Pastoral; Fortunato Sevilla
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Paper Abstract

A new antioxidant activity assay based on the reactive oxygen species (ROS)-inducible bacterial strain (E. coli DPD2511) is described. The strain harbors the plasmid pKatG::luxCDABE and responds to hydrogen peroxide treatment by increasing light emission at 490 nm. Antioxidant capacity is evaluated through the ability of an agent to inhibit the hydrogen peroxide-induced bioluminescence of E. coli DPD2511. Applicability of the developed assay in detecting levels of antioxidants in various aqueous plant extracts is demonstrated. The assay was validated against 2,2-diphenylpicrylhydrazyl (DPPH) assay, a known antioxidant assay.

Paper Details

Date Published: 23 February 2006
PDF: 8 pages
Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980J (23 February 2006); doi: 10.1117/12.643950
Show Author Affiliations
Amelita Bartolome, Univ. of Santo Tomas (Philippines)
Research Ctr. for the Natural Sciences (Philippines)
Bernadette Macalino, Univ. of Santo Tomas (Philippines)
Ian Lemuel Pastoral, Univ. of Santo Tomas (Philippines)
Fortunato Sevilla, Univ. of Santo Tomas (Philippines)
Research Ctr. for the Natural Sciences (Philippines)

Published in SPIE Proceedings Vol. 6098:
Genetically Engineered Probes for Biomedical Applications
Alexander P. Savitsky; Rebekka M. Wachter, Editor(s)

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