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Proceedings Paper

Plant antigenotoxicity assay using red fluorescent protein (RFP)-expressing bacteria
Author(s): Katheryn Mandap; Kevim David; Fortunato Sevilla III; Amelita Bartolome
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Paper Abstract

Analytical biophotonics techniques such as steady-state fluorescence spectroscopy and fluorescence microscopy have been proven to be important tools in the study of genetically engineered bacterial sensors for plant antigenotoxicity. The assay involves the use of E. coli RS4U tagged with the red fluorescent protein (RFP). The cells emit red fluoresce in direct proportion to the genotoxicant present. Antigenotoxicity is seen as an act of preventing the DNA-damage induced expression of RFP. Thus, co-treatment of the cells with the genotoxicant and antigenotoxicant plant samples resulted to the reduction of the RFP fluorescence.

Paper Details

Date Published: 23 February 2006
PDF: 8 pages
Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980I (23 February 2006); doi: 10.1117/12.643928
Show Author Affiliations
Katheryn Mandap, Univ. of Santo Tomas (Philippines)
Kevim David, Univ. of Santo Tomas Research Ctr. for Natural Sciences (Philippines)
Fortunato Sevilla III, Univ. of Santo Tomas (Philippines)
Univ. of Santo Tomas Research Ctr. for Natural Sciences (Philippines)
Amelita Bartolome, Univ. of Santo Tomas (Philippines)
Univ. of Santo Tomas Research Ctr. for Natural Sciences (Philippines)


Published in SPIE Proceedings Vol. 6098:
Genetically Engineered Probes for Biomedical Applications
Alexander P. Savitsky; Rebekka M. Wachter, Editor(s)

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