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Proceedings Paper

Simultaneous excitation of multiple fluororophores with a compact femtosecond laser
Author(s): Philippe Legros; Daniel Choquet; Stéphane Gueguen; Eric Mottay; Nelly Deguil
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Paper Abstract

We report on a flexible multiphoton imaging system, suitable for simultaneous and efficient excitation of red (DsRed), yellow (YFP), green (GFP) and blue (DAPI) fluorophores. We used a simple, compact laser system, consisting of a 1 μm high energy diode-pumped oscillator and a tunable wavelength extension using a photonics crystal fibre. The combination of a near IR excitation wavelength, high energy per pulse for efficient three photon excitation and spectral extension for GFP excitation allows for high flexibility.We present experimental results of simultaneous and efficient imaging fluorophores couples from the UV to the red (DAPI-RFP, GFP-RFP).

Paper Details

Date Published: 23 February 2006
PDF: 6 pages
Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60890U (23 February 2006); doi: 10.1117/12.643774
Show Author Affiliations
Philippe Legros, Univ. Bordeaux II (France)
Daniel Choquet, Univ. Bordeaux II (France)
Stéphane Gueguen, Amplitude Systemes (France)
Eric Mottay, Amplitude Systemes (France)
Nelly Deguil, Amplitude Systemes (France)


Published in SPIE Proceedings Vol. 6089:
Multiphoton Microscopy in the Biomedical Sciences VI
Ammasi Periasamy; Peter T. C. So, Editor(s)

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