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Proceedings Paper

In vitro and in vivo on-chip biofluorescence imaging using a CMOS image sensor
Author(s): David C. Ng; Masamichi Matsuo; Takashi Tokuda; Keiichiro Kagawa; Masahiro Nunoshita; Jun Ohta
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Paper Abstract

We have designed and fabricated a 176×144-pixels (QCIF) CMOS image sensor for on-chip bio-fluorescence imaging of the mouse brain. In our approach, a single CMOS image sensor chip without additional optics is used. This enables imaging at arbitrary depths into the brain; a clear advantage compared to existing optical microscopy methods. Packaging of the chip represents a challenge for in vivo imaging. We developed a novel packaging process whereby an excitation filter is applied onto the sensor. This eliminates the use of a filter cube found in conventional fluorescence microscopes. The fully packaged chip is about 350 μm thick. Using the device, we demonstrated in vitro on-chip fluorescence imaging of a 400 μm thick mouse brain slice detailing the hippocampus. The image obtained compares favorably to the image captured by conventional microscopes in terms of image resolution. In order to study imaging in vivo, we also developed a phantom media. In situ fluorophore measurement shows that detection through the turbid medium of up to 1 mm thickness is possible. We have successfully demonstrated imaging deep into the hippocampal region of the mouse brain where quantitative fluorometric measurements was made. This work is expected to lead to a promising new tool for imaging the brain in vivo.

Paper Details

Date Published: 6 February 2006
PDF: 9 pages
Proc. SPIE 6068, Sensors, Cameras, and Systems for Scientific/Industrial Applications VII, 606801 (6 February 2006); doi: 10.1117/12.642591
Show Author Affiliations
David C. Ng, Nara Institute of Science and Technology (Japan)
Masamichi Matsuo, Nara Institute of Science and Technology (Japan)
Takashi Tokuda, Nara Institute of Science and Technology (Japan)
Keiichiro Kagawa, Nara Institute of Science and Technology (Japan)
Masahiro Nunoshita, Nara Institute of Science and Technology (Japan)
Jun Ohta, Nara Institute of Science and Technology (Japan)


Published in SPIE Proceedings Vol. 6068:
Sensors, Cameras, and Systems for Scientific/Industrial Applications VII
Morley M. Blouke, Editor(s)

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