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Proceedings Paper

Optofluidic microscope and its applications in biology
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Paper Abstract

In this article, we will demonstrate a novel optical imaging device that can be directly integrated into a microfluidic network, and therefore enables on-chip imaging in a microfluidic system. This micro imaging device, termed optofluidic microscope (OFM) is potentially free of bulk optics and is based on a nanohole array defined in a nontransmissive metallic layer that is patterned onto the floor of the microfluidic channel. The operation of the optofluidic microscope will be explained in details and its performance is examined by using a popular animal model, Caenorhabditis elegans (C. elegans). Images from a large population of nematode worms are efficiently acquired within a short time frame. The quality of the OFM images of C. elegans and the morphological characteristics revealed therein are evaluated. Two groups of early-stage C elegans larvae, wild-type and dpy-24 are successfully separated even though their morphological difference at the larval stage is subtle. The experimental results support our claim that the methodology described therein can be effectively used to develop a powerful tool for fulfilling high-resolution, highthroughput imaging task in microfluidics-based systems.

Paper Details

Date Published: 21 February 2006
PDF: 9 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608816 (21 February 2006); doi: 10.1117/12.640325
Show Author Affiliations
Xin Heng, California Institute of Technology (United States)
Kevin W. Reynolds, Norfolk State Univ. (United States)
Xiquan Cui, California Institute of Technology (United States)
David Erickson, Cornell Univ. (United States)
Demetri Psaltis, California Institute of Technology (United States)
Changhuei Yang, California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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