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Proceedings Paper

Cellular vitality of PC12 cells revealed through auto-fluorescence induced by two-photon excitation
Author(s): Elena Perevedentseva; Artashes Karmenyan; Fu-Jen Kao
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Paper Abstract

The combination of fluorescence spectroscopy and optical trapping under an integrated laser scanning microscopy platform has presented to be a powerful tool in manipulating and positioning the cells as well as standardizing the conditions of measurements. This platform was applied to investigate the autofluorescence of PC12 cells. Under 2-p excitation, the PC12 cells exhibit fluorescence in the range of 500-530 nm. It was shown that intensity of 2-p autofluorescence from PC12 cells is very sensitive to the cells' condition. Therefore autofluorescence from the cells may be a convenient and noninvasive method for monitoring the cell's or another biological object's condition, metabolism, and/or vitality under various physical and chemical influences.

Paper Details

Date Published: 7 December 2006
PDF: 10 pages
Proc. SPIE 5973, Current Research on Laser Use in Oncology: 2000-2004, 597311 (7 December 2006); doi: 10.1117/12.640220
Show Author Affiliations
Elena Perevedentseva, National Dong-Hwa Univ. (Taiwan)
P.N. Lebedev Physics Institute, RAS (Russia)
Artashes Karmenyan, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
National Sun Yat-Sen Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5973:
Current Research on Laser Use in Oncology: 2000-2004
Andrei V. Ivanov; Mishik A. Kazaryan, Editor(s)

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