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Changes in the aqueous phase of cell suspensions and protein solutions induced by a photoexitation of the molecular oxygen (to mechanisms of biological action of low level laser radiation)
Author(s): S. D. Zakharov; A. V. Ivanov
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Paper Abstract

Temperature-dependent transient processes initiated by a direct photogeneration of singlet oxygen in suspensions of human erythrocytes and solutions of serum albumin are studied. The processes appear as anomalous jumps in the temperature dependences of the deformability coefficient of erythrocytes and the refractive index of the extracellular medium and protein solution. In the temperature regions of anomalous jumps, cells and proteins transfer to a metastable state of a lower activity, but they can be isothermally photoreactivated. Simultaneously, a reversible rearrangement of the aqueous phase occurs near the cell and protein surfaces, accompanied by the formation of an extended corona (hydrogel). The transient processes are interpreted as phase transitions in the membrane of erythrocytes and conformation transitions in proteins. The interaction between erythrocytes and albumin via hydrogel is discovered (hydro-conformational interaction).

Paper Details

Date Published: 7 December 2006
PDF: 14 pages
Proc. SPIE 5973, Current Research on Laser Use in Oncology: 2000-2004, 59730V (7 December 2006); doi: 10.1117/12.640184
Show Author Affiliations
S. D. Zakharov, P.N. Lebedev Physical Institute, RAS (Russia)
A. V. Ivanov, N.N. Blokhin Russian Cancer Research Ctr., RAMS (Russia)

Published in SPIE Proceedings Vol. 5973:
Current Research on Laser Use in Oncology: 2000-2004
Andrei V. Ivanov; Mishik A. Kazaryan, Editor(s)

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