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Proceedings Paper

Measurement of erythrocyte deformability by the flow-channel diffraction method
Author(s): A. Górnicki; A. Kempczyński
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Paper Abstract

Erythrocyte deformability, known as ability of red blood cell (RBC) to change shape during flow in the microcirculation, is the basic rheological property of erythrocyte. Many techniques have been proposed to measure RBC's deformability (e.g. micropipette aspiration, micropore filtration, high shear viscometer and rheoscope techniques). Perhaps one of the most elegant technique for measurements of RBC deformability is ektacytometry, technique based on laser diffraction, worked out by Bessis and Mohandas1 and recently developed by others2,3. The laser light scattered by erythrocytes subjected to a well-defined shear stress (z) can be analyzed with the ektacytometer to obtain information regarding the changes in cell shape due to fluid shear. Usually, in order to deform RBC with shear stress the rotating Couette chamber is used 1-5. From the diffraction images the elongation index (E), as a quotient of minor and major axes of the elliptically transformed diffraction patterns, is calculated. However, one has to face a series of methodological problems 5: 1. Occurrence of turbulences and heat shear, 2. Control of gap width within the Couette viscometer and the intensity of transmitted light, 3. The determination of RBC elongation coefficients E from the diffraction patterns. Therefore, in order to get accurate results, we have used unconventional technique which consists of a simple laminar flow system made from two parallel Plexiglas plates (flow-channel diffractometer) and the computer analysis of the diffraction pattern.

Paper Details

Date Published: 6 December 2006
PDF: 4 pages
Proc. SPIE 5945, 14th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 59451K (6 December 2006); doi: 10.1117/12.639042
Show Author Affiliations
A. Górnicki, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)
A. Kempczyński, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)

Published in SPIE Proceedings Vol. 5945:
14th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Anton Štrba; Dagmar Senderákova; Miroslav Hrabovský, Editor(s)

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