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Proceedings Paper

Cell separation technique in dilectrophoretic chip with bulk electrode
Author(s): Ciprian Iliescu; Francis E. H. Tay; Guolin Xu; Liming Yu
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Paper Abstract

This paper presents a new technique for separation of two cell populations in a dielectrophoretic chip with bulk silicon electrode. A characteristic of the dielectrophoretic chip is its "sandwich" structure: glass/silicon/glass that generates a unique definition of the microfluidic channel with conductive walls (silicon) and isolating floor and ceiling (glass). The structure confers the opportunity to use the electrodes not only to generate a gradient of the electric field but also to generate a gradient of velocity of the fluid inside the channel. This interesting combination gives rise to a new solution for dielectrophoretic separation of two cell populations. The separation method consists of four steps. First, the microchannel is field with the cells mixture. Second, the cells are trapped in different locations of the microfluidic channel, the cell population which exhibits positive dielectrophoresis is trapped in the area where the distance between the electrodes is the minimum whilst, the other population that exhibit negative dielectrophoresis is trapped where the distance between electrodes is the maximum. In the next step, increasing the flow in the microchannel will result in an increased hydrodynamic force that sweeps the cells trapped by positive dielectrophoresis out of the chip. In the last step, the electric field is removed and the second population is sweep out and collected at the outlet. The device was tested for separation of dead yeast cells from live yeast cells. The paper presents analytical aspects of the separation method a comparative study between different electrode profiles and experimental results.

Paper Details

Date Published: 19 January 2006
PDF: 12 pages
Proc. SPIE 6036, BioMEMS and Nanotechnology II, 60360E (19 January 2006); doi: 10.1117/12.638509
Show Author Affiliations
Ciprian Iliescu, Institute of Bioengineering and Nanotechnology (Singapore)
Francis E. H. Tay, Institute of Bioengineering and Nanotechnology (Singapore)
National Univ. of Singapore (Singapore)
Guolin Xu, Institute of Bioengineering and Nanotechnology (Singapore)
Liming Yu, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 6036:
BioMEMS and Nanotechnology II
Dan V. Nicolau, Editor(s)

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