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Proceedings Paper

UV-VIS extinction spectra of gold particle coated by oligonucleotide shell
Author(s): Vladimir A. Bogatyrev; Stanislav A. Vrublevsky; Lyubov A. Trachuk; Nikolai G. Khlebtsov
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Paper Abstract

We describe synthesis process of an oligonucleotide-functionalized colloidal gold marker CG-l5-T28, its optical properties and interaction with poly(A) in solution and on a solid-phase substrate. The marker is a complex of 15 nm diameter colloidal gold nanoparticles with covalently attached 5'-thiolated 28-base oligothymidine macromolecules. A positive hybridization reaction of the marker with poly(A) is observed by solid-phase analysis on hanging a spot color (from red to blue ) or on appearance of a red dye in dot-blot test as compared to control experiments with poly(U) target. The principles of spectrophotometric monitoring all stages of the marker preparation and application of spectrophotometry to detection of the polynucleotide hybridization in vitro are described. Experimental data were compared with theoretical calculations based on Mie theory for 2-layer model of gold core in polymeric shell with imaginary part of refractive index that typical for the real absorption spectra of NA. To explain the aggregation of CG-15-T28 caused by interaction with poly(A) in solution, we suggest a new model differing from a standard model of cross-linker binding.

Paper Details

Date Published: 9 June 2005
PDF: 7 pages
Proc. SPIE 5772, Saratov Fall Meeting 2004: Coherent Optics of Ordered and Random Media V, (9 June 2005); doi: 10.1117/12.636776
Show Author Affiliations
Vladimir A. Bogatyrev, Institute of Biochemistry and Physiology of Plants and Microorganisms, RAS (Russia)
Saratov State Univ. (Russia)
Stanislav A. Vrublevsky, Saratov State Univ. (Russia)
Lyubov A. Trachuk, Institute of Biochemistry and Physiology of Plants and Microorganisms, RAS (Russia)
Nikolai G. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms, RAS (Russia)
Saratov State Univ. (Russia)


Published in SPIE Proceedings Vol. 5772:
Saratov Fall Meeting 2004: Coherent Optics of Ordered and Random Media V
Dmitry A. Zimnyakov, Editor(s)

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